Alena Hanusova scored 15 points as the Czech Republic beat Serbia 65-62 to capture the bronze medal at the Termosteps U16 European Championship Woman, increasing their U16 medal total to three.
The 1997 and 2006 finalists won other medals in the past, but they came under the Czechoslovakia national flag - including the 1989 gold.
"This feeling is impossible to describe. During the whole tournament we struggled but we finally played how I wanted them to. The Serbia team was very good, but my team wanted to win more than they did," said Czech coach Richard Fousek.
"Czech women's basketball long-term is among the top six places in Europe. And this is a confirmation of the talent of the junior teams. This is a great satisfaction for all the hard work during this competition."
Czech center Gabriela Medunova added: "It's a fantastic feeling and I'm so proud of what we did. I'm sure this shows everybody that Czech basketball is very good and we can win more medals in the future."
Serbia led early 15-11 after the first quarter and increased their advantage to 22-14. But the Czechs were ahead 31-30 at the break. Fousek's team led throughout the third quarter by as many as six points.
The Serbs evened the game at 52-52 and again at 60-60 with 1:46 remaining. But after a Lenka Bartakova layup, Serbia turned over the ball and Hanusova's foul shot opened a 65-62 lead with 6.7 seconds left.
Serbia turned over the ball once again as Marina Markovic couldn't handle Jasna Vasilev's pass with 2.9 seconds to go, deciding the game.
Adela Filarova scored 13 points for the Czechs and Bartakova chipped in 11.
Sara Krnjic had 14 points, 14 rebounds and four blocked shots and Tina Jovanovic added 15 points and 11 rebounds in a losing effort for Serbia.